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KMID : 0352819930090010001
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Kosin Medical Journal
1993 Volume.9 No. 1 p.1 ~ p.22
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Immuohistochemical Invesitgation For Distribution of Fibronectin in the Early Developing Moues Embryos
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Abstract
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It is known that the organogenesis is established as a result of migration of three germ cell layers in the early stage of embryogenesis. In an attempt to examine the distribution and expression patterns of fibronectin, immunhistochemical
localization
was performed.
Mouse embryos of gestational age 6 to 13 days were observed. Paraffinembedded sections were immunostained with antibody to fibronectin(indirect Avidin-Biotin peroxidase method).
1. in the early stage of blastocyst(6th day mouse embryo), intracellular expression of fibronectin was observed in primitive endodermal cella of the inner cell mass. However, positivity for intracellular fibronectin diminished as gestational age
increased.
2. Extracellular expression of fibronectin was detected in the interstitiaispaces of primitive endodermal cells prior to diferentiation and was noted on the inner surface of trophoblast and extraembryonic endoderm.
3. Extracelluar fibronectin was also reactive in intervening spaces of each germ cell layer.
4. In addition, extraembryonic membrance(chorion and yolk sac wall) expressed fibronectin.
These findings may support the theory that fibronectin is esssential to the proper location of cells in thh early developing embryo.
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KEYWORD
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